Having training intraspecific variation away from recombination speed for the maize, i authored a couple of 50 % of-sib panels, one having Drop plus one to have Flint inbred lines. Drop and you may Flint are a couple of maize gene pools which can be essential having hybrid breeding from inside the Europe . Both 50 % of-sib panels composed a total of twenty four complete-sib family members. New Drop committee comprises 10 crosses away from a main Damage inbred line (F353) which have varied Dent inventor inbreds of one’s European Damage gene pond which were developed in Europe and The united states. On Flint committee a main Flint inbred range (UH007) is crossed to help you 11 naturally diverse Flint maker inbreds of the European Flint breeding question. The Flint originator contours result from early maize introductions to help you The country of spain together with off thus-titled Northern Flints regarding France and Germany. On top of that, one or two mutual crosses among them central Dent and you will Flint outlines was analyzed plus for each panel one get across on Us Drop line B73 is actually incorporated since a connection to the us nested connection mapping (NAM) population .
Here, we report on the analysis of the variation of recombination rate in 23 populations of maize using SNPs from the MaizeSNP50 BeadChip . All populations consist of doubled haploid (DH) lines obtained by in vivo haploid induction . DH lines produced with this method reflect female meiosis. Unlike F2 or RILs, DH lines have the great advantage that the genetic information of each gamete is directly observed. Our objectives were to analyze intraspecific variation for (1) GWRR and chromosome-wide recombination rate, (2) recombination landscape along chromosomes, and (3) CO interference, using high-density genetic linkage maps. We found significant differences of GWRR between individual populations as well as between Dent ? Dent versus Flint ? Flint populations, but not between the Dent and Flint gene pools in general. GWRR was not correlated with the genetic structure of the lines as inferred from admixture analysis. We analyzed the recombination landscapes in all populations and found significant differences between individual populations and between pools. Finally, we characterized quantitatively the interfering and non-interfering CO formation pathways and found a negative correlation over all chromosomes between interference intensity of pathway P1 and GWRR.
Show
We constructed two half-sib panels comprising a total of 24 maize full-sib families of DH lines for the analysis of intraspecific variation of recombination rates. The full-sib families within the two panels represent the diversity of important founder lines of the European Dent and Flint germplasm, respectively (Table 1; Additional file 1). In the Dent panel (prefix CFD), all full-sib families have the same common parental line F353 which was crossed to diverse Dent founder lines. The common parent of the Flint full-sib families (prefix CFF) is line UH007, which was crossed to diverse Flint founder lines. 1 plants for in vivo haploid induction, so our maps reflect only female meioses. Details on the diversity analysis of the parental lines are given in Additional file 2.
In every crosses, the haploid inducer range was applied because men father or mother when entered into F
Once the a first step in the study of recombination, individual hereditary maps were developed to have 23 out of the twenty-four communities and each of 10 chromosomes. Map analytics are supplied during the Dining table step 1 with more info during the Extra file 3, plus the done variety of marker positions in all charts having the new brutal segregation data is given into the Extra file cuatro. Overall, 39,439 SNPs of the MaizeSNP50 range will be mapped from inside the during the least 
you to society. Total populations, up to 33,884 COs was indeed found in dos,233 women meioses and, typically, 11,988 SNPs was indeed mapped in a single offered populace. The common to possess Dent ? Drop populations (the CFD except CFD01) try thirteen,247 markers mapped as well as Flint ? Flint populations (all of the CFF except CFF01 and you will CFF02) the typical try 9,874. To have inhabitants CFF05, which manufactured just 34 DHs, no stable chart might be obtained. The entire duration of hereditary charts ranged from one,180 centiMorgan (cM) for the inhabitants CFD05 to a single,893 cM for the CFF03 with a suggest of just one,508 ± 185 cM (indicate ± simple deviation). The average hereditary map size on ten Dent ? Drop populations (CFD02 so you’re able to CFD12) is 1,353 ± 99 cM, since the eleven hereditary maps off Flint ? Flint populations (CFF03 to CFF15) had been, an average of, longer at the step 1,645 ± 154 cM. The 3 maps regarding crosses ranging from Reduction and you will Flint mothers (CFD01, CFF01, CFF02) had an advanced average duration of step one,570 ± 85 cM. The greatest pit inside a genetic chart are 53.eight cM into the long-arm regarding chromosome 7 (7L) inside inhabitants CFF13, where no SNPs was basically polymorphic involving the moms and dads, most likely due to label-by-ancestry (IBD). Details about genome-wide variety and you can IBD locations ranging from mothers of your own mapping populations are located in A lot more file 2. Synteny and you may colinearity of your own genetic charts is weighed against this new B73 AGPv2 genome set up. Overall, a high arrangement is receive, showing the newest highest consistency regarding marker acquisition all over communities, that have pair exclusions due to the fact discussed in the Extra file 2.
